#GreenChemistry

A long-standing scientific debate has finally been resolved. Researchers have confirmed a “crazy” theory from 1958 about vitamin B1, or thiamine. By stabilizing an extremely reactive carbene intermediate in water, they observed a stable form long thought impossible. The breakthrough, published in Science Advances, signals new possibilities for sustainable chemical manufacturing worldwide, including in Thailand.

The idea dates back to Ronald Breslow at Columbia University, who proposed that vitamin B1 forms a fleeting carbene-like species during essential cellular reactions. Carbenes are notoriously unstable in water, the solvent of life, which kept the theory unproven for decades. Breslow’s concept seemed almost magical to many scientists.

In a scientific breakthrough decades in the making, researchers have finally confirmed a “crazy” theory dating back to 1958 involving vitamin B1, or thiamine. By successfully stabilizing an extremely reactive molecule known as a carbene in water—something scientists long believed to be impossible—this team has not only resolved a long-standing biochemical debate but also unlocked new possibilities for sustainable chemical manufacturing around the world, including Thailand. Their results, published in Science Advances on April 11, 2025, have sparked fresh excitement in both academic and industrial laboratories worldwide (SciTechDaily).

A historic breakthrough confirms a 67-year-old hypothesis about vitamin B1, or thiamine, revealing new details about how this essential nutrient operates in the body. The finding not only resolves a long-standing scientific debate but also opens possibilities for eco-friendly chemical production with potential benefits for health, industry, and the environment.

The discovery traces back to 1958, when a Columbia University chemist proposed that thiamine’s metabolic actions might involve a reactive structure called a carbene. Carbenes are usually unstable in water-based environments, making such a mechanism seem unlikely for biological processes. The idea faced skepticism for decades until a UC Riverside-led team demonstrated otherwise by stabilizing a carbene in water. This achievement paved the way for observing the molecule’s composition with high-resolution imaging, marking the first verified instance of a stable carbene in aqueous conditions.

In a historic breakthrough, scientists have finally confirmed a 67-year-old hypothesis about vitamin B1, also known as thiamine, radically advancing our understanding of how this crucial vitamin works in the human body. This finding not only settles a decades-old scientific debate but also unlocks new possibilities for eco-friendly chemical production, with potential impact reaching far beyond the laboratory.

The story of this discovery begins in 1958, when a chemist from Columbia University theorized that vitamin B1 might perform its vital metabolic functions through the formation of an elusive molecular structure called a carbene. Carbenes are notoriously unstable and tend to decompose instantly in aqueous (water-based) environments like the human body, making the hypothesis seem improbable for more than half a century. For years, the idea was dismissed as “crazy,” as quoted by Vincent Lavallo, a chemist at the University of California, Riverside, who led the recent research that validated this bold proposal.